Description
Module to build the VDJ reference needed by the 10x Genomics Cell Ranger tool. Uses the cellranger mkvdjref command.
Tools
cellranger Documentation
Cell Ranger processes data from 10X Genomics Chromium kits. cellranger vdj
takes FASTQ files from cellranger mkfastq
or bcl2fastq
for V(D)J libraries and performs sequence assembly and paired clonotype calling. It uses the Chromium cellular barcodes and UMIs to assemble V(D)J transcripts per cell. Clonotypes and CDR3 sequences are output as a .vloupe
file which can be loaded into Loupe V(D)J Browser.