Description
A program that counts sequence occurrences in FASTQ files.
Tools
2FAST2Q Documentation
2FAST2Q is ideal for CRISPRi-Seq, and for extracting and counting any kind of information from reads in the fastq format, such as barcodes in Bar-seq experiments. 2FAST2Q can work with sequence mismatches, Phred-score, and be used to find and extract unknown sequences delimited by known sequences. 2FAST2Q can extract multiple features per read using either fixed positions or delimiting search sequences.
doi: 10.7717/peerj.14041License: GPL-3.0-or-later