Description

NACHO (NAnostring quality Control dasHbOard) is developed for NanoString nCounter data. NanoString nCounter data is a messenger-RNA/micro-RNA (mRNA/miRNA) expression assay and works with fluorescent barcodes. Each barcode is assigned a mRNA/miRNA, which can be counted after bonding with its target. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA.

Input

Name
Description
Pattern

0 ()

1 ()

0 ()

1 ()

Output

Name
Description
Pattern

normalized_counts ()

normalized_counts_wo_HK ()

versions ()

Tools

NACHO Documentation

R package that uses two main functions to summarize and visualize NanoString RCC files, namely: load_rcc() and visualise(). It also includes a function normalise(), which (re)calculates sample specific size factors and normalises the data. For more information vignette("NACHO") and vignette("NACHO-analysis")

doi: 10.1093/bioinformatics/btz647License: GPL-3.0
Authors