Description

NACHO (NAnostring quality Control dasHbOard) is developed for NanoString nCounter data. NanoString nCounter data is a messenger-RNA/micro-RNA (mRNA/miRNA) expression assay and works with fluorescent barcodes. Each barcode is assigned a mRNA/miRNA, which can be counted after bonding with its target. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA.

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Tools

NACHO Documentation

R package that uses two main functions to summarize and visualize NanoString RCC files, namely: load_rcc() and visualise(). It also includes a function normalise(), which (re)calculates sample specific size factors and normalises the data. For more information vignette("NACHO") and vignette("NACHO-analysis")

doi: 10.1093/bioinformatics/btz647License: GPL-3.0
Authors