Description

Filter the per-read-length P-site offset table down to the (length, offset) pairs that will actually drive ORF-level scoring. Drops read lengths whose metagene profile is too sparsely populated, or whose periodicity Bayes factor is too low / too uncertain, so that downstream P-site counting only uses read lengths with a clean 3-nucleotide signal.

Wraps Rp-Bp's get_periodic_lengths_and_offsets Python helper directly. Thresholds are configured via named flags in ext.args: --min-count (default: 1000), --min-bf-mean (default: 5), --max-bf-var (default: no limit), --min-bf-likelihood (default: 0.5). Defaults mirror rpbp.defaults.metagene_options.

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Tools

rpbp Documentation

Rp-Bp - Bayesian inference of ribosome profiling data for identifying translated open reading frames