Description
Pick the single best P-site offset for each read length from the per-(length, offset) Bayes factor table produced upstream. For each read length, the offset with the highest periodicity Bayes-factor mean is selected - this is the offset that, when added to a read's 5' end, is estimated to land closest to the ribosomal P-site (the codon being decoded). Downstream, these offsets are used to convert raw read 5'-end coordinates into P-site positions when counting reads against candidate ORFs.
Emits one row per read length (length, best offset, supporting Bayes
factor statistics). The next step
(rpbp/getperiodiclengthsoffsets) filters this table to the high-quality
pairs that pass user-specified count / signal thresholds before
P-site counting in rpbp/extractorfprofiles.
Tools
rpbp Documentation
Rp-Bp - Bayesian inference of ribosome profiling data for identifying translated open reading frames
Command copied!