Description

Pick the single best P-site offset for each read length from the per-(length, offset) Bayes factor table produced upstream. For each read length, the offset with the highest periodicity Bayes-factor mean is selected - this is the offset that, when added to a read's 5' end, is estimated to land closest to the ribosomal P-site (the codon being decoded). Downstream, these offsets are used to convert raw read 5'-end coordinates into P-site positions when counting reads against candidate ORFs.

Emits one row per read length (length, best offset, supporting Bayes factor statistics). The next step (rpbp/getperiodiclengthsoffsets) filters this table to the high-quality pairs that pass user-specified count / signal thresholds before P-site counting in rpbp/extractorfprofiles.

Input

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Output

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Tools

rpbp Documentation

Rp-Bp - Bayesian inference of ribosome profiling data for identifying translated open reading frames