Parameters
Path/URL to one assembly FASTA file to analyse.
Supports .fa/.fasta and .fa.gz/.fasta.gz. Use this for single-assembly runs instead of --input.
Path to a CSV samplesheet describing one or more assemblies.
Expected header is fasta,taxid,mode,lineage,outdir. Row-level values override global --taxid/--mode/--lineage for that assembly.
pattern: ^\S+\.csv$Global NCBI taxid for lineage discovery.
Used when samplesheet taxid is not provided. Required for modes ancestral/latest.
Global explicit BUSCO lineage(s) to add.
Comma-separated lineage names (e.g. mammalia,insecta). Used when samplesheet lineage is empty.
Directory containing BUSCO taxid mapping files.
Files must be named
Local BUSCO database directory (optional).
If set, selected lineages are validated locally and BUSCO runs in offline mode.
Global lineage discovery mode(s): basal, latest, ancestral.
Comma-separated values allowed. latest and ancestral require taxid and cannot be combined together.
ODB version(s) to use.
Comma-separated values (odb10,odb12,odb12.2). Each listed version requires a corresponding mapping file in mapping_directory.
The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.
Email address for completion summary.
Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (~/.nextflow/config) then you don't need to specify this on the command line for every run.
pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$MultiQC report title. Printed as page header, used for filename if not otherwise specified.
Use Nextflow work directory for temporary files where supported.
Parameters used to describe centralised config profiles. These should not be edited.
The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.
Git commit id for Institutional configs.
default: 'master'Base directory for Institutional configs.
default: 'https://raw.githubusercontent.com/nf-core/configs/master'If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.
Less common options for the pipeline, typically set in a config file.
These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.
Typically these options would be set in a Nextflow config file loaded for all pipeline runs, such as ~/.nextflow/config.
Method used to save pipeline results to output directory.
The Nextflow publishDir option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See Nextflow docs for details.
Email address for completion summary, only when pipeline fails.
An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.
pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$File size limit when attaching MultiQC reports to summary emails.
default: '25.MB'Incoming hook URL for messaging service
Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.
Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file
Custom MultiQC yaml file containing HTML including a methods description.
Boolean whether to validate parameters against the schema at runtime
default: 1Base URL or local path to location of pipeline test dataset files
default: 'https://raw.githubusercontent.com/nf-core/test-datasets/'Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.