Parameters

Input/output options

Define where the pipeline should find input data and save output data.

--input

type: 'string'

Path to comma-separated file containing information about the samples in the experiment.

required

You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.

pattern: ^\S+\.csv$

--scientific_name

type: 'string'

Scientific name of sample according to NCBI

--sample_id

type: 'string'

Sample ID

--taxid

type: 'integer'

TaxID according to NCBI

--reads_path

type: 'array'

folder containing long read data files in FASTA.GZ format

--reads_type

type: 'string'

Type of read (hifi)

--reads_layout

type: 'string'

Specify whether all reads are SINGLE or PAIRED end reads

--pacbio_barcode_file

type: 'string'

Fasta format file containing barcode data

--pacbio_barcode_names

type: 'string'

barcodes to use in run

--kmer_length

type: 'number'

Length of kmer to use in run

--dimensionality_reduction_methods

type: 'array'

dimensionality_reduction_methods to use in runs

--nt_database_path

type: 'string'

folder containing nt database

--nt_database_prefix

type: 'string'

Prefix of the NT database

--nt_kraken_database_path

type: 'string'

folder containing Kraken2 database

--ncbi_taxonomy_path

type: 'string'

folder containing dmp files

--ncbi_ranked_lineage_path

type: 'string'

Path to rankedlineage.dmp

--busco_lineages_folder

type: 'string'

BUSCO folder containing busco Lineages folder

--busco_lineages

type: 'string'

CSV list of lineages to use in run

--fcs_gx_database_path

type: 'string'

FCS_GX folder containing database

--vecscreen_database_path

type: 'string'

Vecscreen folder containing database

--diamond_uniprot_database_path

type: 'string'

Path to Uniprot Diamond DB

--diamond_nr_database_path

type: 'string'

Path to NR Diamond DB

--seqkit_sliding

type: 'number'

Length of slide for Seqkit

--seqkit_window

type: 'number'

Length of the window for Seqkit

--n_neighbours

type: 'number'

Nearest Neighbour value - This is for the dimensional reduction of kmer counts (UMAP and t-SNE).

--btk_yaml

type: 'string'

Path to BTK yaml

--outdir

type: 'string'

The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.

required

--email

type: 'string'

Email address for completion summary.

Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (~/.nextflow/config) then you don't need to specify this on the command line for every run.

pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

--multiqc_title

type: 'string'

MultiQC report title. Printed as page header, used for filename if not otherwise specified.

--include

type: 'string'

Select steps to run.

--exclude

type: 'string'

Deselect steps to run.

--organellar_include

type: 'string'

Select steps to run - specific to the organallar workflow.

--organellar_exclude

type: 'string'

Deselect steps to run - specific to the organallar workflow.

--btk_busco_run_mode

type: 'string'

Should btk be run as mandatory or conditional on levels of contamination.

Institutional config options

Parameters used to describe centralised config profiles. These should not be edited.

hidden

The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.

--custom_config_version

type: 'string'

Git commit id for Institutional configs.

default: 'master'
hidden

--custom_config_base

type: 'string'

Base directory for Institutional configs.

default: 'https://raw.githubusercontent.com/nf-core/configs/master'
hidden

If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.

--config_profile_name

type: 'string'

Institutional config name.

hidden

--config_profile_description

type: 'string'

Institutional config description.

hidden

--config_profile_contact

type: 'string'

Institutional config contact information.

hidden

--config_profile_url

type: 'string'

Institutional config URL link.

hidden

Generic options

Less common options for the pipeline, typically set in a config file.

These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.

Typically these options would be set in a Nextflow config file loaded for all pipeline runs, such as ~/.nextflow/config.

--version

type: 'boolean'

Display version and exit.

hidden

--publish_dir_mode

type: 'string'

Method used to save pipeline results to output directory.

The Nextflow publishDir option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See Nextflow docs for details.

--email_on_fail

type: 'string'

Email address for completion summary, only when pipeline fails.

hidden

An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.

pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

--plaintext_email

type: 'boolean'

Send plain-text email instead of HTML.

hidden

--max_multiqc_email_size

type: 'string'

File size limit when attaching MultiQC reports to summary emails.

default: '25.MB'
hidden

--monochrome_logs

type: 'boolean'

Do not use coloured log outputs.

hidden

--hook_url

type: 'string'

Incoming hook URL for messaging service

hidden

Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.

--multiqc_config

type: 'string'

Custom config file to supply to MultiQC.

hidden
type: 'string'

Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file

hidden

--multiqc_methods_description

type: 'string'

Custom MultiQC yaml file containing HTML including a methods description.

--validate_params

type: 'boolean'

Boolean whether to validate parameters against the schema at runtime

default: 1
hidden

--pipelines_testdata_base_path

type: 'string'

Base URL or local path to location of pipeline test dataset files

default: 'https://raw.githubusercontent.com/nf-core/test-datasets/'
hidden

--trace_report_suffix

type: 'string'

Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.

hidden

The following uncommon parameters have been hidden: --custom_config_version, --custom_config_base, --config_profile_name, --config_profile_description, --config_profile_contact, --config_profile_url, --version, --publish_dir_mode, --email_on_fail, --plaintext_email, --max_multiqc_email_size, --monochrome_logs, --hook_url, --multiqc_config, --multiqc_logo, --validate_params, --pipelines_testdata_base_path, --trace_report_suffix

to show all hidden params.